Sio
Anime name	Sio
Manga name	Sio
Alternate names	Lady Sio The Number One Headband, The #1
Debut	Afro Samurai: Resurrection
Appears in	Afro Samurai: Resurrection
Race	Human (Japanese)
Gender	Female
Address	Dharma's Laboratory
Occupation	Samurai
Allegiance	Headband Code, Dharma, Jinno
Likes	Jinno, her loved ones
Dislikes	Afro Samurai
Family	Jinno (brother)

Sio is the younger sister of Kuma/Jinno and the main antagonist in Afro Samurai: Resurrection. In Afro Samurai: Resurrection she convinces Kuma/Jinno to attack Afro. Once she and Kuma finished him they took the Number One Headband and the jaw of Afro's father.

Sio then proceeded to resurrect Rokutaro (prematurely), and then had him fight Afro. He then killed Afro to the delight of Sio. Kuma/Jinno realized that what they had done was wrong made an attempt to kill Rokutaro, but was easily dispatched and subsequently mutilated by Rokutaro. Sio tried to save him but was killed along with him, due to Kuma/Jinno having been used as a shield by Rokutaro.

Sio liked Afro as a child, but due to the murder of her adopted family she has a resentment towards him. Sio subsequently blames Afro for the murder of her adopted family even though he was not directly linked to their death.